Application
Immunohistochemistry of EFCAB4B in human kidney tissue with EFCAB4B antibody at 10 μg/mL.Application
Immunofluorescence of EFCAB4B in human kidney tissue with EFCAB4B antibody at 20 μg/mL.Application
Western blot analysis of EFCAB4B in mouse kidney tissue lysate with EFCAB4B antibody at 1 μg/mL.| Product Name | EFCAB4B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EFCAB4B Antibody: CRACR2A, CRACR2A, EF-hand calcium-binding domain-containing protein 4B, Calcium release-activated calcium channel regulator 2A, CRAC channel regulator 2A |
| Product description | EFCAB4B Antibody: EFCAB4B, also known as Calcium release-activated calcium channel regulator 2A, is a novel Ca2+-binding EF-hand protein that is thought to play a key role in store-operated Ca2+ entry in T-cells by regulating CRAC channel activation. EFCAB4B acts as a cytoplasmic calcium-sensor that forms a complex with ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+ concentration. A closely related protein, EFCAB4A, is likely to play a similar role as EFCAB4B, but the detailed function of EFCAB4A is still under investigation.1) Srikanth S, Jung HJ, Kim KD, et al. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell. Biol.2010; 12:436-46. |
| Immunogen | EFCAB4B antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human EFCAB4B. The immunogen is located within amino acids 640 - 690 of EFCAB4B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| EFCAB4B antibody can be used for detection of EFCAB4B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFCAB4B |
|---|---|
| Alternative Names | EFCAB4B Antibody: CRACR2A CRACR2A EF-hand calcium-binding domain-containing protein 4B Calcium release-activated calcium channel regulator 2A CRAC channel regulator 2A |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Entrez Gene | 84766 |
|---|---|
| Protein Accession | NP_001138430 |
Application
Immunohistochemistry of EFCAB4B in human kidney tissue with EFCAB4B antibody at 10 μg/mL.
Application
Immunofluorescence of EFCAB4B in human kidney tissue with EFCAB4B antibody at 20 μg/mL.
Application
Western blot analysis of EFCAB4B in mouse kidney tissue lysate with EFCAB4B antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | EFCAB4B antibody can be used for detection of EFCAB4B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EFCAB4B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EFCAB4B Antibody is supplied in PBS containing 0.02% sodium azide. |
