Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of EFEMP2 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)Application
Western blot analysis of EFEMP2 Antibody in HepG2 cell line lysates (35ug/lane).| Product Name | EFEMP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EGF-containing fibulin-like extracellular matrix protein 2, Fibulin-4, FIBL-4, Protein UPH1, EFEMP2, FBLN4 |
| Product description | EFEMP2 have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein contains four EGF2 domains and six calcium-binding EGF2 domains. This protein is necessary for elastic fiber formation and connective tissue development.1) Chen,Q., et.al., Biochem. J. 423 (1), 79-89 (2009) |
| Immunogen | This EFEMP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-414 amino acids from the C-terminal region of human EFEMP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFEMP2 |
|---|---|
| Alternative Names | EGF-containing fibulin-like extracellular matrix protein 2 Fibulin-4 FIBL-4 Protein UPH1 EFEMP2 FBLN4 |
| Molecular Weight(MW) | 49 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Hamster |
Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of EFEMP2 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
Application
Western blot analysis of EFEMP2 Antibody in HepG2 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
