Application
Immunofluorescence of EFHD1 in rat spleen tissue with EFHD1 antibody at 5 μg/mL.Application
Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 μg/mL.| Product Name | EFHD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EFHD1 Antibody: SWS2, MST133, PP3051, MSTP133, SWS2, EF-hand domain-containing protein D1, EF-hand domain-containing protein 1 |
| Product description | EFHD1 Antibody: EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.1) Tominaga M and Tomooka Y. Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53-/- mouse. Biochem. Biophys. Res. Comm.2002; 297:473-9. |
| Immunogen | EFHD1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1. The immunogen is located within the first 50 amino acids of EFHD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| EFHD1 antibody can be used for detection of EFHD1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFHD1 |
|---|---|
| Alternative Names | EFHD1 Antibody: SWS2 MST133 PP3051 MSTP133 SWS2 EF-hand domain-containing protein D1 EF-hand domain-containing protein 1 |
Application
Immunofluorescence of EFHD1 in rat spleen tissue with EFHD1 antibody at 5 μg/mL.
Application
Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | EFHD1 antibody can be used for detection of EFHD1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EFHD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EFHD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
