Application
Immunohistochemistry of EFHD2 in human brain tissue with EFHD2 antibody at 5 μg/mL.Application
Immunofluorescence of EFHD2 in human brain tissue with EFHD2 antibody at 20 μg/mL.Application
Western blot analysis of EFHD2 in mouse brain tissue lysate with EFHD2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | EFHD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EFHD2 Antibody: SWS1, SWS1, EF-hand domain-containing protein D2, Swiprosin-1 |
| Product description | EFHD2 Antibody: EFHD2, also known as Swiprosin-1 or SWS1, is an EF-hand and coiled-coil-containing adaptor protein that plays a role in lymphocyte physiology. EFHD2 exhibits the highest expression in CD8+ T cells and immature B cells. It provides a membrane scaffold that is required for the Syk-, SLP-65-, and PLCgamma2-dependent B-cell receptor (BCR)-induced calcium flux. EFHD2 may also regulate BCR-induced immature and primary B-cell apoptosis. It controls spontaneous apoptosis through the regulation of BCL2L1 abundance. Also, EFHD2 plays a role as negative regulator of the canonical NF-κB-activating branch.1) Kroczek C, Lang C, Brachs S, et al. Swiprosin-1/EFhd2 controls B cell receptor signaling through the assembly of the B cell receptor, Syk, and phospholipase C gamma2 in membrane rafts. J. Immunol.2010; 184:3665-76. |
| Immunogen | EFHD2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human EFHD2. The immunogen is located within the first 50 amino acids of EFHD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| EFHD2 antibody can be used for detection of EFHD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFHD2 |
|---|---|
| Alternative Names | EFHD2 Antibody: SWS1 SWS1 EF-hand domain-containing protein D2 Swiprosin-1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of EFHD2 in human brain tissue with EFHD2 antibody at 5 μg/mL.
Application
Immunofluorescence of EFHD2 in human brain tissue with EFHD2 antibody at 20 μg/mL.
Application
Western blot analysis of EFHD2 in mouse brain tissue lysate with EFHD2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | EFHD2 antibody can be used for detection of EFHD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EFHD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EFHD2 Antibody is supplied in PBS containing 0.02% sodium azide. |
