Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 ug/ml – 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 ug/ml – 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 ug/ml – 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.Application
To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human EGF (XP-5119Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.| Product Name | EGF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | URG, HOMG4Pro-epidermal growth factor, Urogastrone, EGF |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/mL), a concentration of 0.25 0.35 μg/mL of this antibody is required. ELISA: To detect hEGF by direct ELISA (using 100us antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Sandwich: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human EGF (XP-5119Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGF |
|---|---|
| Alternative Names | URG HOMG4Pro-epidermal growth factor Urogastrone EGF |
| Molecular Weight(MW) | 6.2 kDa |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 ug/ml – 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 ug/ml – 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 ug/ml – 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Application
To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human EGF (XP-5119Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/mL), a concentration of 0.25 0.35 μg/mL of this antibody is required. ELISA: To detect hEGF by direct ELISA (using 100us antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Sandwich: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human EGF (XP-5119Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | EGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
