Application
Western Blot at 1:2000 dilution Lane 1: M.stomach tissue lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western blot analysis in mouse stomach tissue lysates (35ug/lane).Application
EGF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Antibody staining EGF in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Antibody staining EGF in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | EGF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF |
| Product description | This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.1) de Diesbach, M.T., et al. Exp. Cell Res. 316(19):3239-3253(2010) Xu, Z., et al. Biochem. Biophys. Res. Commun. 401(3):376-381(2010) Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010) Hommel, U., et al. J. Mol. Biol. 227(1):271-282(1992) Hernandez-Sotomayor, S.M., et al. J. Membr. Biol. 128(2):81-89(1992) |
| Immunogen | This EGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-720 amino acids from the Central region of human EGF. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:200 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGF |
|---|---|
| Alternative Names | Pro-epidermal growth factor EGF Epidermal growth factor Urogastrone EGF |
| Molecular Weight(MW) | 134 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: M.stomach tissue lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in mouse stomach tissue lysates (35ug/lane).
Application
EGF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Antibody staining EGF in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Antibody staining EGF in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:200 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
