Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | EHD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EH domain-containing protein 1, PAST homolog 1, hPAST1, Testilin, EHD1, PAST, PAST1 |
| Product description | This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors.1) Gudmundsson, H., et al. Circ. Res. 107(1):84-95(2010) |
| Immunogen | This EHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-438 amino acids from the C-terminal region of human EHD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EHD1 |
|---|---|
| Alternative Names | EH domain-containing protein 1 PAST homolog 1 hPAST1 Testilin EHD1 PAST PAST1 |
| Molecular Weight(MW) | 61 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
