Ehf is a transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. It may act as a repressor for a specific subset of ETS/AP-1-responsive genes, and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. It binds to DNA sequences containing the consensus nucleotide core sequence GGAA and involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter1) Ko, M.S., (2000) Development 127 (8), 1737-1749.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse Ehf.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
Ehf antibody can be used for detection of Ehf by ELISA at 1:312500. Ehf antibody can be used for detection of Ehf by western blot at 1.0¦ μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse Prostate at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human kidney.
Positive Control
1) Mouse Prostate Lysate
Application Notes
Ehf antibody can be used for detection of Ehf by ELISA at 1:312500. Ehf antibody can be used for detection of Ehf by western blot at 1.0¦ μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store Ehf antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.