Application
Immunohistochemistry of EI24 in rat liver tissue with EI24 antibody at 5 μg/mL.Application
Western blot analysis of EI24 in rat liver tissue lysate with EI24 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | EI24 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EI24 Antibody: EPG4, PIG8, TP53I8, p53-induced gene 8 protein |
| Product description | EI24 Antibody: The etoposide-induced 24 (EI24) protein is an immediate-early induction target of p53-mediated apoptosis and functions as a tumor suppressor. EI24 contains six transmembrane domains and is localized to the endoplasmic reticulum (ER). It binds to Bcl-2 and may contribute to apoptosis by modulating the activity and function of Bcl-2. EI24 is highly mutated in aggressive breast cancers and its loss in breast cancer tissue correlates with tumor invasiveness. Similar findings in invasive cervical carcinoma suggest that EI24 may be a promising therapeutic target in the treatment of invasive cancer.1) Lehar SM, Nacht M, Jacks T, et al. Identification and cloning of EI24, a gene induced by p53 in etoposide-treated cells. Oncogene 1996; 12:1181-7. |
| Immunogen | EI24 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human EI24. The immunogen is located within the last 50 amino acids of EI24. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| EI24 antibody can be used for detection of EI24 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EI24 |
|---|---|
| Alternative Names | EI24 Antibody: EPG4 PIG8 TP53I8 p53-induced gene 8 protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (95%) |
| Tissue Specificity | At least two isoforms of EI24 are known to exist; this antibody will only detect the larger isoform. |
Application
Immunohistochemistry of EI24 in rat liver tissue with EI24 antibody at 5 μg/mL.
Application
Western blot analysis of EI24 in rat liver tissue lysate with EI24 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | EI24 antibody can be used for detection of EI24 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EI24 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EI24 Antibody is supplied in PBS containing 0.02% sodium azide. |
