Application
Antibody used in WB on Human HEK293T cells at 1 ug/ml.Application
Antibody used in WB on Human Hela at 1 ug/ml.Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Product Name | EIF2AK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF2AK1, HRI, KIAA1369, HCR |
| Product description | EIF2AK1 acts at the level of translation initiation to downregulate protein synthesis in response to stress. The protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat and rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Sequence Note: The sequence AF181071.1 is a chimeric mRNA clone. Only the heme-regulated initiation factor 2-alpha kinase region was propagated into this RefSeq record.1) Olsen, J.V., (2006) Cell 127 (3), 635-648. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2AK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| EIF2AK1 antibody can be used for detection of EIF2AK1 by ELISA at 1:62500. EIF2AK1 antibody can be used for detection of EIF2AK1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2AK1 |
|---|---|
| Alternative Names | EIF2AK1 HRI KIAA1369 HCR |
| Molecular Weight(MW) | 71 kDa |
Application
Antibody used in WB on Human HEK293T cells at 1 ug/ml.
Application
Antibody used in WB on Human Hela at 1 ug/ml.
Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10410 - Fetal Lung Tissue Lysate |
|---|---|
| Application Notes | EIF2AK1 antibody can be used for detection of EIF2AK1 by ELISA at 1:62500. EIF2AK1 antibody can be used for detection of EIF2AK1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF2AK1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
