Application
E immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of E for immunohistochemistry.Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the EIF2B4 antibody detected the EIF2B4 protein (arrow).Application
Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2B4 antibody detected the EIF2B4 protein (arrow).| Product Name | EIF2B4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Translation initiation factor eIF-2B subunit delta, eIF-2B GDP-GTP exchange factor subunit delta, EIF2B4, EIF2BD |
| Product description | Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].1) Naukkarinen, J., et al. PLoS Genet.(6), E1000976 (2010) : |
| Immunogen | This EIF2B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human EIF2B4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2B4 |
|---|---|
| Alternative Names | Translation initiation factor eIF-2B subunit delta eIF-2B GDP-GTP exchange factor subunit delta EIF2B4 EIF2BD |
| Molecular Weight(MW) | 58 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
E immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of E for immunohistochemistry.
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the EIF2B4 antibody detected the EIF2B4 protein (arrow).
Application
Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2B4 antibody detected the EIF2B4 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
