Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
EIF2C2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2C2 antibody detected the EIF2C2 protein (arrow).| Product Name | EIF2C2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein argonaute-2 {ECO:0000255|HAMAP-Rule:MF_03031}, Argonaute2 {ECO:0000255|HAMAP-Rule:MF_03031}, mAgo2, 3126n2 {ECO:0000255|HAMAP-Rule:MF_03031}, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2 {ECO:0000255|HAMAP-Rul |
| Product description | EIF2C2 is a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.1) Rivas, F.V., et al., Nat Struct Mol Biol 12(4):340-349 (2005). |
| Immunogen | This EIF2C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-59 amino acids from the N-terminal region of human EIF2C2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ago2 |
|---|---|
| Alternative Names | Protein argonaute-2 {ECO:0000255|HAMAP-Rule:MF_03031} Argonaute2 {ECO:0000255|HAMAP-Rule:MF_03031} mAgo2 3126n2 {ECO:0000255|HAMAP-Rule:MF_03031} Argonaute RISC catalytic component 2 Eukaryotic translation initiation factor 2C 2 {ECO:0000255|HAMAP-Rul |
| Molecular Weight(MW) | 97 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
EIF2C2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2C2 antibody detected the EIF2C2 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
