Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.| Product Name | EIF4E2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF4E2, 4EHP, IF4e, 4E-LP, EIF4EL3 |
| Product description | EIF4E2 belongs to the eukaryotic initiation factor 4E family. It recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.1) Tee, A.R., FEBS Lett. 564 (1-2), 58-62 (2004). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4E2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| EIF4E2 antibody can be used for detection of EIF4E2 by ELISA at 1:62500. EIF4E2 antibody can be used for detection of EIF4E2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EIF4E2 |
|---|---|
| Alternative Names | EIF4E2 4EHP IF4e 4E-LP EIF4EL3 |
| Molecular Weight(MW) | 27 kDa |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | EIF4E2 antibody can be used for detection of EIF4E2 by ELISA at 1:62500. EIF4E2 antibody can be used for detection of EIF4E2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF4E2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
