Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of EIF4E2 using rabbit polyclonal EIF4E2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.Application
Western blot analysis of EIF4E2 Antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | EIF4E2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mR |
| Product description | EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.1) Rom, E., et al., J. Biol. Chem. 273(21):13104-13109 (1998). |
| Immunogen | This EIF4E2 antibody is generated from rabbits immunized with human EIF4E2 recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4E2 |
|---|---|
| Alternative Names | Eukaryotic translation initiation factor 4E type 2 eIF-4E type 2 eIF4E type 2 Eukaryotic translation initiation factor 4E homologous protein Eukaryotic translation initiation factor 4E-like 3 eIF4E-like protein 4E-LP mRNA cap-binding protein 4EHP mR |
| Molecular Weight(MW) | 28 kDa |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of EIF4E2 using rabbit polyclonal EIF4E2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.
Application
Western blot analysis of EIF4E2 Antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
