EIF4E3 belongs to the EIF4E family of translational initiation factors that interact with the 5-prime cap structure of mRNA and recruit mRNA to the ribosome.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4E3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
EIF4E3 antibody can be used for detection of EIF4E3 by ELISA at 1:62500. EIF4E3 antibody can be used for detection of EIF4E3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Application Notes
EIF4E3 antibody can be used for detection of EIF4E3 by ELISA at 1:62500. EIF4E3 antibody can be used for detection of EIF4E3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF4E3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.