Application
Antibody used in WB on Chicken, Turkey at 1:1000.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | EIF4G2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF4G2, P97, AAG1, DAP5, NAT1 |
| Product description | Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. EIF4G2 shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, EIF4G2 functions as a general repressor of translation by forming translationally inactive complexes.1) NIH MGC Project, (2001) Direct Submission. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4G2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| EIF4G2 antibody can be used for detection of EIF4G2 by ELISA at 1:312500. EIF4G2 antibody can be used for detection of EIF4G2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4G2 |
|---|---|
| Alternative Names | EIF4G2 P97 AAG1 DAP5 NAT1 |
| Molecular Weight(MW) | 39 kDa, 98 kDa, 102 kDa |
Application
Antibody used in WB on Chicken, Turkey at 1:1000.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | EIF4G2 antibody can be used for detection of EIF4G2 by ELISA at 1:312500. EIF4G2 antibody can be used for detection of EIF4G2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF4G2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
