Application
Immunohistochemistry staining of EIF4G2 in brain cortex tissue using EIF4G2 monoclonal Antibody.| Product Name | EIF4G2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF4G2, AAG1, Aging-associated protein 1, DAP-5, DAP5, Death-associated protein 5, EIF-4G 2, EIF-4-gamma 2, EIF4G 2, Translation repressor nat1, p97 |
| Product description | Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death. A fragment of the cDNA when expressed as a mini-protein of 28 kDa protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97 kDa. Low level of expression protects the cells, whereas high level of expression results in cell death (3-4). |
| Immunogen | EIF4G2 monoclonal antibody was raised against amino acids 796 - 812 of EIF4G2 (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| EImmunofluorescence4G2 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | EIF4G2 |
|---|---|
| Alternative Names | EIF4G2 AAG1 Aging-associated protein 1 DAP-5 DAP5 Death-associated protein 5 EIF-4G 2 EIF-4-gamma 2 EIF4G 2 Translation repressor nat1 p97 |
| Tissue Specificity | In Jurkat, a 97 kD band is observed. |
Application
Immunohistochemistry staining of EIF4G2 in brain cortex tissue using EIF4G2 monoclonal Antibody.| Application Notes | EImmunofluorescence4G2 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | EIF4G2 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.2% gelatin, 0.05% sodium azide. |
