Application
Confocal immunofluorescent analysis of EIF5AL1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in Ramos,A2058 cell line lysates (35ug/lane).Application
EIF5AL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | EIF5AL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Eukaryotic translation initiation factor 5A-1-like, eIF-5A-1-like, eIF-5A1-like, Eukaryotic initiation factor 5A isoform 1-like, EIF5AL1 |
| Product description | EIF5AL1is a mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).1) Kim, S.C., et al. Mol. Cell 23(4):607-618(2006) |
| Immunogen | This EIF5AL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the C-terminal region of human EIF5AL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF5AL1 |
|---|---|
| Alternative Names | Eukaryotic translation initiation factor 5A-1-like eIF-5A-1-like eIF-5A1-like Eukaryotic initiation factor 5A isoform 1-like EIF5AL1 |
| Molecular Weight(MW) | 17 kDa |
Application
Confocal immunofluorescent analysis of EIF5AL1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in Ramos,A2058 cell line lysates (35ug/lane).
Application
EIF5AL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
