Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Product Name | ELF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ELF4, MEF, ELFR |
| Product description | ELF4 contains 1 ETS DNA-binding domain and belongs to the ETS family. It is transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. It transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. It acts synergistically with RUNX1 to transactivate the IL3 promoter and also transactivates the PRF1 promoter in natural killer (NK) cells. ELF4 plays a role in the development and function of NK and NK T-cells and in innate immunity.1) Cho, J.Y., (2004) J. Biol. Chem. 279 (19), 19512-19522. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ELF4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELF4 antibody can be used for detection of ELF4 by ELISA at 1:312500. ELF4 antibody can be used for detection of ELF4 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ELF4 |
|---|---|
| Alternative Names | ELF4 MEF ELFR |
| Molecular Weight(MW) | 71 kDa |
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | ELF4 antibody can be used for detection of ELF4 by ELISA at 1:312500. ELF4 antibody can be used for detection of ELF4 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ELF4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
