Application
Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).Application
Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ELF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ETS-related transcription factor Elf-4, E74-like factor 4, Myeloid Elf-1-like factor, ELF4 {ECO:0000312|EMBL:CAI428821} |
| Product description | ELF4 is transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. The protein acts synergistically with RUNX1 to transactivate the IL3 promoter. Also transactivates the PRF1 promoter in natural killer (NK) cells. This protein plays a role in the development and function of NK and NK T-cells and in innate immunity.1) Miyazaki Y., Sun X.Oncogene 13:1721-1729(1996) |
| Immunogen | This ELF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-237 amino acids from the Central region of human ELF4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ELF4 |
|---|---|
| Alternative Names | ETS-related transcription factor Elf-4 E74-like factor 4 Myeloid Elf-1-like factor ELF4 {ECO:0000312|EMBL:CAI428821} |
| Molecular Weight(MW) | 71 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
