Application
Immunohistochemical analysis of paraffin - embedded human brain tumor tissue showing nuclear and cytoplasmic location using ELK1 antibody with DAB staining.Application
Western blot analysis using ELK1 monoclonal antibody against truncated ELK1 recombinant protein and K562 cell lysate.| Product Name | ELK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ETS domain-containing protein Elk-1 |
| Product description | The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.1) Rao,V.N., et al. 1989.Science.244 (4900):66-70. |
| Immunogen | Ni-NTA purified truncated recombinant ELK1 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:1,000 IHC(P):1:500 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ELK1 |
|---|---|
| Alternative Names | ETS domain-containing protein Elk-1 |
Application
Immunohistochemical analysis of paraffin - embedded human brain tumor tissue showing nuclear and cytoplasmic location using ELK1 antibody with DAB staining.
Application
Western blot analysis using ELK1 monoclonal antibody against truncated ELK1 recombinant protein and K562 cell lysate.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:1,000 IHC(P):1:500 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ELK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
