Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).Application
Western blot analysis of Elongin A using rabbit polyclonal Elongin A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Elongin A gene.| Product Name | Elongin A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription elongation factor B polypeptide 3, Elongin 110 kDa subunit, Elongin-A, EloA, RNA polymerase II transcription factor SIII subunit A1, SIII p110, TCEB3 |
| Product description | Elongin A is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.1) Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004). |
| Immunogen | This Elongin A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-127 amino acids from the N-terminal region of human Elongin A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCEB3 |
|---|---|
| Alternative Names | Transcription elongation factor B polypeptide 3 Elongin 110 kDa subunit Elongin-A EloA RNA polymerase II transcription factor SIII subunit A1 SIII p110 TCEB3 |
| Molecular Weight(MW) | 90 kDa |
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Application
Western blot analysis of Elongin A using rabbit polyclonal Elongin A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Elongin A gene.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
