Epithelial membrane protein-2 (EMP2) is a member of the four transmembrane superfamily (TM4SF) and is thought to mediate trafficking of diverse proteins such as alpha6beta1 integrin and MHC class I to lipid raft microdomains. EMP2 has also recently been recognized as a putative tumor suppressor gene in certain model systems.1) Wadehra, M., (2005) Dev. Biol. 287 (2), 336-345.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EMP2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
EMP2 antibody can be used for detection of EMP2 by ELISA at 1:312500. EMP2 antibody can be used for detection of EMP2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
EMP2 antibody can be used for detection of EMP2 by ELISA at 1:312500. EMP2 antibody can be used for detection of EMP2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EMP2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt