Application
Immunohistochemistry of EndoG in human pancreas tissue with EndoG antibody at 2.5 μg/ml.Application
Immunohistochemistry of EndoG in human pancreas tissue with EndoG antibody at 2.5 μg/ml.Application
Immunohistochemistry of EndoG in human pancreas with EndoG antibody at 15 μg/mL.Application
Western blot analysis of EndoG in mouse (M) 3T3 and human (H) HepG2 cell lysates with EndoG antibody at 2 μg/mL.| Product Name | EndoG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EndoG Antibody: Endonuclease G, mitochondrial, Endo G |
| Product description | EndoG Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.1) Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature. 2001;412(6842):95-9. |
| Immunogen | EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG. The immunogen is located within amino acids 40 - 90 of EndoG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| EndoG antibody can be used for detection of EndoG by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ENDOG |
|---|---|
| Alternative Names | EndoG Antibody: Endonuclease G mitochondrial Endo G |
| Molecular Weight(MW) | 35 kDa |
| Sequence Similarities | 4) Cat. No. 11-101 - Human Pancreas Tissue Slide |
Application
Immunohistochemistry of EndoG in human pancreas tissue with EndoG antibody at 2.5 μg/ml.
Application
Immunohistochemistry of EndoG in human pancreas tissue with EndoG antibody at 2.5 μg/ml.
Application
Immunohistochemistry of EndoG in human pancreas with EndoG antibody at 15 μg/mL.
Application
Western blot analysis of EndoG in mouse (M) 3T3 and human (H) HepG2 cell lysates with EndoG antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | EndoG antibody can be used for detection of EndoG by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EndoG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EndoG Antibody is supplied in PBS containing 0.02% sodium azide. |
