Application
Western blot analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 2.5 and (B) 5 μg/mL.| Product Name | EndoG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EndoG Antibody [7G1C10] : Endo G |
| Product description | EndoG Monoclonal Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans . EndoG also cleaves DNA in vitro.1) Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9. |
| Immunogen | Recombinant protein corresponding to amino acids 51 - 140 of human EndoG. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| EndoG monoclonal antibody can be used for detection of EndoG by Western blot at 2.5 - 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ENDOG |
|---|---|
| Alternative Names | EndoG Antibody [7G1C10] : Endo G |
| Sequence Similarities | 2) Cat. No. 95-103 - EndoG Recombinant Protein |
Application
Western blot analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 2.5 and (B) 5 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | EndoG monoclonal antibody can be used for detection of EndoG by Western blot at 2.5 - 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EndoG monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | EndoG Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
