Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Product Name | ENO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ENO1, NNE, PPH, MPB1, ENO1L1 |
| Product description | ENO1 is one of three enolase isoenzymes found in mammals; its gene encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.1) Ghosh, A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ENO1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ENO1 antibody can be used for detection of ENO1 by ELISA at 1:62500. ENO1 antibody can be used for detection of ENO1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | ArabidopsisC.elegansHumanZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ENO1 |
|---|---|
| Alternative Names | ENO1 NNE PPH MPB1 ENO1L1 |
| Molecular Weight(MW) | 47 kDa |
Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ENO1 antibody can be used for detection of ENO1 by ELISA at 1:62500. ENO1 antibody can be used for detection of ENO1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ENO1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
