Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).Application
ENPEP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ENPEP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glutamyl aminopeptidase, EAP, Aminopeptidase A, AP-A, Differentiation antigen gp160, CD249, ENPEP |
| Product description | ENPEP appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.1) Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010) |
| Immunogen | This ENPEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 899-930 amino acids from the C-terminal region of human ENPEP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ENPEP |
|---|---|
| Alternative Names | Glutamyl aminopeptidase EAP Aminopeptidase A AP-A Differentiation antigen gp160 CD249 ENPEP |
| Molecular Weight(MW) | 109 kDa |
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Application
ENPEP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
