Application
(LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ENTH Antibody in HL-60 cell line lysates (35ug/lane)| Product Name | ENTH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Clathrin interactor 1, Clathrin-interacting protein localized in the trans-Golgi region, Clint, Enthoprotin, Epsin-4, Epsin-related protein, EpsinR, CLINT1, ENTH, EPN4, EPNR, KIAA0171 |
| Product description | ENTH binds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). It May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. It stimulates clathrin assembly.1) Richards,M., et.al., J. Neural Transm. 115 (9), 1347-1354 (2008) |
| Immunogen | This ENTH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-249 amino acids from the Central region of human ENTH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CLINT1 |
|---|---|
| Alternative Names | Clathrin interactor 1 Clathrin-interacting protein localized in the trans-Golgi region Clint Enthoprotin Epsin-4 Epsin-related protein EpsinR CLINT1 ENTH EPN4 EPNR KIAA0171 |
| Molecular Weight(MW) | 68 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
(LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ENTH Antibody in HL-60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
