Application
Immunohistochemistry of EPAC3 in mouse liver tissue with EPAC3 antibody at 2.5 μg/mL.Application
Immunofluorescence of EPAC2 in mouse liver tissue with EPAC2 antibody at 20 μg/mL.Application
Western blot analysis of EPAC2 in rat liver tissue lysate with EPAC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | EPAC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EPAC2 Antibody: 2, EPAC, CGEF2, EPAC2, EPAC 2, Nbla00496, CAMP-GEFII, Rap guanine nucleotide exchange factor 4, Exchange factor directly activated by cAMP 2 |
| Product description | EPAC2 Antibody: EPAC2, also known as Rap guanine nuclear exchange factor 4 and cAMPGEF-II, is belongs to a family of cyclic adenosine monophosphate (cAMP) binding proteins with guanine nucleotide exchange factor. Like the related protein EPAC1, EPAC2 signaling plays a role in numerous cellular processes such as integrin-mediated cell adhesion, muscle contraction, learning and memory, cell proliferation, and inflammation. Recent evidence suggests that EPAC2 induces synapse remodeling and depression, with mutations in its gene being found in patients with autism.1) Ueno H, Shibasaki T, Iwanaga T, et al. Characterization of the gene EPAC2: structure, chromosomal localization, tissue expression, and identification of the liver-specific isoform. Genomics 2001; 78:91-8 |
| Immunogen | EPAC2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human EPAC2. The immunogen is located within the first 50 amino acids of EPAC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| EPAC2 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAPGEF4 |
|---|---|
| Alternative Names | EPAC2 Antibody: 2 EPAC CGEF2 EPAC2 EPAC 2 Nbla00496 CAMP-GEFII Rap guanine nucleotide exchange factor 4 Exchange factor directly activated by cAMP 2 |
| Tissue Specificity | At least two isoforms of EPAC2 are known to exist; this antibody will detect only the larger isoform. EPAC2 antibody is predicted to not cross-react with EPAC2 |
Application
Immunohistochemistry of EPAC3 in mouse liver tissue with EPAC3 antibody at 2.5 μg/mL.
Application
Immunofluorescence of EPAC2 in mouse liver tissue with EPAC2 antibody at 20 μg/mL.
Application
Western blot analysis of EPAC2 in rat liver tissue lysate with EPAC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | EPAC2 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EPAC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EPAC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
