Erythrocyte membrane protein band 4.2 is an ATP-binding protein which may regulate the association of protein 3 with ankyrin. It probably has a role in erythrocyte shape and mechanical property regulation. Mutations in the EPB42 gene are associated with recessive spherocytic elliptocytosis and recessively transmitted hereditary hemolytic anemia.1) Dahl, K.N., et al., (2004) Blood 103 (3), 1131-1136.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EPB42.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
EPB42 antibody can be used for detection of EPB42 by ELISA at 1:1562500. EPB42 antibody can be used for detection of EPB42 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Application Notes
EPB42 antibody can be used for detection of EPB42 by ELISA at 1:1562500. EPB42 antibody can be used for detection of EPB42 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EPB42 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.