Application
Immunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody.Application
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | EPHA 2/3/4 (phospho Tyr588/596) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ECK, MPK-5, SEK2, kinase EphA2 |
| Product description | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.1) Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990). |
| Immunogen | EPHA 2/3/4 (phospho Tyr588/596) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y (p)-V-R)/ (R-T-Y (p)-V-D) derived from Human EPHA2/3/4. |
| Modification | p-Tyr588/596 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EPHA2, EPHA3, EPHA4 |
|---|---|
| Alternative Names | ECK MPK-5 SEK2 kinase EphA2 |
| Molecular Weight(MW) | 130 kDa |
| Tissue Specificity | The antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596 |
| Entrez Gene | 1969/2042/2043 |
|---|---|
| Protein Accession | NP_004422.2 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody.
Application
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
