Application
Western blot analysis of EphB3 Antibody in mouse stomach tissue lysates (35ug/lane)Application
Flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of EphB3 Antibody in C6 cell line lysates (35ug/lane)| Product Name | EphB3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ephrin type-B receptor 3, EPH-like tyrosine kinase 2, EPH-like kinase 2, Embryonic kinase 2, EK2, hEK2, Tyrosine-protein kinase TYRO6, EPHB3, ETK2, HEK2, TYRO6 |
| Product description | Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB3, a member of the Tyr family of protein kinases, is a receptor for members of the ephrin-B family; it binds to both ephrin-B1 and -B2. Expression of this Type I membrane protein is ubiquitous. The protein contains putative domains for 2 fibronectin type III and 1 sterile alpha motif (SAM).1) Chiu,S.T., et.al., Carcinogenesis 30 (9), 1475-1486 (2009) |
| Immunogen | This EphB3 antibody is generated from rabbits immunized with EphB3 his fusion protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | EPHB3 |
|---|---|
| Alternative Names | Ephrin type-B receptor 3 EPH-like tyrosine kinase 2 EPH-like kinase 2 Embryonic kinase 2 EK2 hEK2 Tyrosine-protein kinase TYRO6 EPHB3 ETK2 HEK2 TYRO6 |
| Molecular Weight(MW) | 110 kDa |
Application
Western blot analysis of EphB3 Antibody in mouse stomach tissue lysates (35ug/lane)
Application
Flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of EphB3 Antibody in C6 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
