Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Ephrin B1/B2/B3 (phospho Tyr324) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EPL2, CEL5-L, EFL-3, EFNB1, ELK-L |
| Product description | This gene encodes a member of the ephrin family. The encoded protein is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system.1) Beckmann M.P., EMBO J. 13:3757-3762(1994). |
| Immunogen | Ephrin B1/B2/B3 (phospho Tyr324) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 324 (G-D-Y (p)-G-H) derived from Human Ephrin B1/B2/B3 . |
| Modification | p-Tyr324 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFNB1, EFNB2, EFNB3 |
|---|---|
| Alternative Names | EPL2 CEL5-L EFL-3 EFNB1 ELK-L |
| Molecular Weight(MW) | 46 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324. |
| Entrez Gene | 1947/1948/1949 |
|---|---|
| Protein Accession | NP_004420.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
