Application
Western blot analysis of lysed extracts from HT29 cells using Ephrin-B2 (Ab-330).Application
Immunofluorescence staining of methanol-fixed HeLa cells using Ephrin-B2 (Ab-330).| Product Name | Ephrin B2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HTKL, EPLG5, Htk-L, LERK5, ON, EFNB2, HTKL, LERK5 |
| Product description | Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.1) Chrencik JE, et al. ( 2006) J Biol Chem;281 (38): 28185-28192. Kertesz N, et al. (2006) Blood; 107 (6): 2330-2338. Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101 (15): 5583-5588. |
| Immunogen | Ephrin-B2 (Ab-330) antibody was raised against a peptide sequence around aa.328~332 (N-I-Y-Y-K) derived from Human Ephrin B (ephrin-B2). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFNB2 |
|---|---|
| Alternative Names | HTKL EPLG5 Htk-L LERK5 ON EFNB2 HTKL LERK5 |
| Molecular Weight(MW) | 37 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Ephrin-B2 protein. |
| Entrez Gene | 1948 |
|---|---|
| Protein Accession | NP_004084.1 |
Application
Western blot analysis of lysed extracts from HT29 cells using Ephrin-B2 (Ab-330).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Ephrin-B2 (Ab-330).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
