Application
Western blot analysis of lysed extracts from HT29 cells, untreated or treated with UV using Ephrin-B2 (Phospho-Tyr316).| Product Name | Ephrin B2 (phospho Tyr316) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HTKL, EPLG5, Htk-L, LERK5, MEK1, PRKMK1, HTKL |
| Product description | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.1) Chrencik JE, et al. ( 2006) J Biol Chem;281 (38): 28185-28192. Kertesz N, et al. ( 2006) Blood;107 (6): 2330-2338. Noren NK, et al. ( 2004) Proc Natl Acad Sci USA;101 (15): 5583-5588 |
| Immunogen | Ephrin-B2 (Phospho-Tyr316) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 316 (P-V-Y (p) -I-V) derived from Human Ephrin-B2. |
| Modification | p-Tyr316 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFNB2 |
|---|---|
| Alternative Names | HTKL EPLG5 Htk-L LERK5 MEK1 PRKMK1 HTKL |
| Molecular Weight(MW) | 37 kDa |
| Tissue Specificity | This antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 316. |
| Entrez Gene | 1948 |
|---|---|
| Protein Accession | NP_004084.1 |
Application
Western blot analysis of lysed extracts from HT29 cells, untreated or treated with UV using Ephrin-B2 (Phospho-Tyr316).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
