Application
Western blot analysis of lysed extracts from HT29 cells, untreated or treated with UV using Ephrin-B2 (Phospho-Tyr330).| Product Name | Ephrin B2 (phospho Tyr330) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HTKL, EPLG5, Htk-L, LERK5, NR1C3, HTKL, MGC126226 |
| Product description | Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.1) Chrencik JE, et al. ( 2006) J Biol Chem;281 (38): 28185-28192. Kertesz N, et al. (2006) Blood; 107 (6): 2330-2338. Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101 (15): 5583-5588. |
| Immunogen | Ephrin-B2 (Phospho-Tyr330) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 330 (N-I-Y (p) -Y-K) derived from Human Ephrin-B2. |
| Modification | p-Tyr330 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFNB2 |
|---|---|
| Alternative Names | HTKL EPLG5 Htk-L LERK5 NR1C3 HTKL MGC126226 |
| Molecular Weight(MW) | 37 kDa |
| Tissue Specificity | This antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 330. |
| Entrez Gene | 1948 |
|---|---|
| Protein Accession | NP_004084.1 |
Application
Western blot analysis of lysed extracts from HT29 cells, untreated or treated with UV using Ephrin-B2 (Phospho-Tyr330).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
