Application
Flow cytometric analysis of MDA-231 cells using EPN3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human colon carcinoma with EPN3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | EPN3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epsin-3, EPS-15-interacting protein 3, EPN3 |
| Product description | EPN3 belongs to the epsin family.1) Spradling,K.D., J. Biol. Chem. 276 (31), 29257-29267 (2001) |
| Immunogen | This EPN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-153 amino acids from the N-terminal region of human EPN3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | EPN3 |
|---|---|
| Alternative Names | Epsin-3 EPS-15-interacting protein 3 EPN3 |
| Molecular Weight(MW) | 68 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of MDA-231 cells using EPN3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma with EPN3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
