Application
Immunohistochemistry of ERAP1 in mouse brain tissue with ERAP1 antibody at 5 μg/mL.Application
Immunofluorescence of ERAP1 in mouse brain tissue with ERAP1 antibody at 20 μg/mL.Application
Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | ERAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERAP1 Antibody : ALAP, A-LAP, ARTS1, ERAAP, APPILS, ARTS-1, ERAAP1, PILSAP, PILS-AP, KIAA0525, UNQ584/PRO1154, Endoplasmic reticulum aminopeptidase 1 |
| Product description | The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1), a member of the peptidase M1 family, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1,2). It is also designated as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1) (3). ERAP1 is localized to the lumen of the ER and induced by interferon. It may be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (3,4).1) Hattori A, Kitatani K, Matsumoto H, et al. Characterization of recombinant human adipocytederived leucine aminopeptidase expressed in Chinese hamster ovary cells. J. Biochem. 2000; 128:755-62. |
| Immunogen | ERAP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERAP. The immunogen is located within amino acids 730 - 780 of ERAP1 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERAP1 |
|---|---|
| Alternative Names | ERAP1 Antibody : ALAP A-LAP ARTS1 ERAAP APPILS ARTS-1 ERAAP1 PILSAP PILS-AP KIAA0525 UNQ584/PRO1154 Endoplasmic reticulum aminopeptidase 1 |
| Molecular Weight(MW) | Predicted: 104 kDa Observed: 105kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (78%) |
| Tissue Specificity | ERAP1 antibody is human and mouse reactive. At least two isoforms of ERAP1 are known to exist; this antibody will detect both isoforms. ERAP1 antibody is predicted to not cross-react with ERAP2. |
Application
Immunohistochemistry of ERAP1 in mouse brain tissue with ERAP1 antibody at 5 μg/mL.
Application
Immunofluorescence of ERAP1 in mouse brain tissue with ERAP1 antibody at 20 μg/mL.
Application
Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ERAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ERAP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
