Application
Western Blot at 1:2000 dilution + U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysates Lane 2: human placenta lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + A549 whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: A549 whole cell lysates Lane 3: NCI-H1299 whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.Application
Antibody staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Antibody staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Overlay histogram showing HepG2 cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goat| Product Name | EREG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proepiregulin, Epiregulin, EPR, EREG |
| Product description | EREG is a member of the epidermal growth factor family. EREG can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors.1) Ben-Ami, I., et al. Hum. Reprod. 24(1):176-184(2009) |
| Immunogen | This EREG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human EREG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EREG |
|---|---|
| Alternative Names | Proepiregulin Epiregulin EPR EREG |
| Molecular Weight(MW) | 19 kDa |
Application
Western Blot at 1:2000 dilution + U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysates Lane 2: human placenta lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: A549 whole cell lysates Lane 3: NCI-H1299 whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Antibody staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Antibody staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Overlay histogram showing HepG2 cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goat| Application Notes | For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
