Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
ERG25 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tisssue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ERG25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Methylsterol monooxygenase 1, C-4 methylsterol oxidase, MSMO1, DESP4, ERG25, SC4MOL |
| Product description | Sterol-C4-mehtyl oxidase-like protein was isolated based on its similarity to the yeast ERG25 protein. It contains a set of putative metal binding motifs with similarity to that seen in a family of membrane desaturases-hydroxylases. The protein is localized to the endoplasmic reticulum membrane and is believed to function in cholesterol biosynthesis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.1) Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008) |
| Immunogen | This ERG25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human ERG25. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MSMO1 |
|---|---|
| Alternative Names | Methylsterol monooxygenase 1 C-4 methylsterol oxidase MSMO1 DESP4 ERG25 SC4MOL |
| Molecular Weight(MW) | 35 kDa |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
ERG25 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tisssue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
