Application
Immunocytochemistry of ERGIC53 in HL60 cells with ERGIC53 antibody at 5 μg/ml.Application
Western blot analysis of ERGIC53 in HL60 cell lysate with ERGIC53 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking buffer.| Product Name | ERGIC53 Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERGIC53 Monoclonal Antibody: Lectin mannose-binding 1, LMAN1, endoplasmic reticulum-golgi intermediate compartment protein 53, ERGIC-53, F5F8D, FMFD1, MCFD1, MR60, gp58 |
| Product description | ERGIC53 Monoclonal Antibody: ERGIC53, also known as lectin mannose-binding 1 (LMAN1) protein is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.1) Schindler R, Itin C, Zerial M, et al. ERGIC-53, a membrane protein of the ER-Golgi intermediate compartment, carries an ER retention motif. Eur. J. Cell Biol. 1993; 61:1-9. |
| Immunogen | Mouse monoclonal ERGIC53 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERGIC53. |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| ERGIC53 antibody can be used for detection of ERGIC53 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LMAN1 |
|---|---|
| Alternative Names | ERGIC53 Monoclonal Antibody: Lectin mannose-binding 1 LMAN1 endoplasmic reticulum-golgi intermediate compartment protein 53 ERGIC-53 F5F8D FMFD1 MCFD1 MR60 gp58 |
| Molecular Weight(MW) | Predicted: 56 kDa |
| Tissue Specificity | At least two isoforms of ERGIC53 are known to exist. |
Application
Immunocytochemistry of ERGIC53 in HL60 cells with ERGIC53 antibody at 5 μg/ml.
Application
Western blot analysis of ERGIC53 in HL60 cell lysate with ERGIC53 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking buffer.| Positive Control | 1) Cat. No. 1209 - HL60 Cell Lysate |
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| Application Notes | ERGIC53 antibody can be used for detection of ERGIC53 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | ERGIC53 Monoclonal antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ERGIC53 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
