Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ERK4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-ERK4 Pab in mouse brain tissue lysate| Product Name | ERK4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase 4, MAP kinase 4, MAPK 4, Extracellular signal-regulated kinase 4, ERK-4, MAP kinase isoform p63, p63-MAPK, MAPK4, ERK4, PRKM4 |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The CMGC group consists of 60 kinases including the cyclin-dependent kinase (CDK) and close relatives family, the MAP kinase (ERK) family, the glycogen synthase kinase 3 (GSK3) family, and the Cdc2-like kinase (CLK) family.1) Robinson, M.J., et al., Curr. Opin. Cell Biol. 9(2):180-186 (1997). |
| Immunogen | This ERK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-557 amino acids from the C-terminal region of human ERK4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | MAPK4 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 4 MAP kinase 4 MAPK 4 Extracellular signal-regulated kinase 4 ERK-4 MAP kinase isoform p63 p63-MAPK MAPK4 ERK4 PRKM4 |
| Molecular Weight(MW) | 66 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ERK4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-ERK4 Pab in mouse brain tissue lysate| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
