Erlin-1 belongs to the band 7/mec-2 family. Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER.1) Browman, D.T., J. Cell. Sci. 119 (PT 15), 3149-3160 (2006).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ERLIN1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
ERLIN1 antibody can be used for detection of ERLIN1 by ELISA at 1:62500. ERLIN1 antibody can be used for detection of ERLIN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in WB on Human 721_B at 1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
ERLIN1 antibody can be used for detection of ERLIN1 by ELISA at 1:62500. ERLIN1 antibody can be used for detection of ERLIN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ERLIN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.