Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
ERO1LB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Product Name | ERO1LB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERO1-like protein beta, ERO1-L-beta, 184-, Endoplasmic reticulum oxidoreductin-1-like protein B, Oxidoreductin-1-L-beta, ERO1LB |
| Product description | ERO1LB is essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1LB acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. ERO1LB does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent.1) Landa, I., et al. PLoS Genet.(9), E1000637 (2009) Savu, M.K., et al. Surg Obes Relat Dis 5(2):172-180(2009) Tavendale, R., et al. J. Allergy Clin. Immunol. 121(4):860-863(2008) |
| Immunogen | This ERO1LB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-293 amino acids from the C-terminal region of human ERO1LB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERO1LB |
|---|---|
| Alternative Names | ERO1-like protein beta ERO1-L-beta 184- Endoplasmic reticulum oxidoreductin-1-like protein B Oxidoreductin-1-L-beta ERO1LB |
| Molecular Weight(MW) | 54 kDa |
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
ERO1LB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
