Application
Flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERP29 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of ERP29 Antibody in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35ug/lane)Application
Western blot analysis of ERP29 using rabbit polyclonal ERP29 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2).| Product Name | ERP29 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Endoplasmic reticulum resident protein 29, ERp29, Endoplasmic reticulum resident protein 28, ERp28, Endoplasmic reticulum resident protein 31, ERp31, ERP29, C12orf8, ERP28 |
| Product description | ERP29 shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.1) Bambang,I.F., et.al., Exp. Cell Res. 315 (11), 1964-1974 (2009) |
| Immunogen | This ERP29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-179 amino acids from the Central region of human ERP29. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ERP29 |
|---|---|
| Alternative Names | Endoplasmic reticulum resident protein 29 ERp29 Endoplasmic reticulum resident protein 28 ERp28 Endoplasmic reticulum resident protein 31 ERp31 ERP29 C12orf8 ERP28 |
| Molecular Weight(MW) | 29 kDa |
Application
Flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERP29 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of ERP29 Antibody in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35ug/lane)
Application
Western blot analysis of ERP29 using rabbit polyclonal ERP29 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
