Application
Immunocytochemistry of ERRF in HeLa cells with ERRF antibody at 2.5 μg/ml.Application
Immunofluorescence of ERRF in HeLa cells with ERRF antibody at 20 μg/ml.Application
Western blot analysis of ERRF in (A) HeLa and (B) A-20 cell lysate with ERRF antibody at 1 μg/mL.| Product Name | ERRF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERRF Antibody: ERRF, Uncharacterized protein C1orf64 |
| Product description | ERRF Antibody: The estrogen receptor-related factor (ERRF) is a recently identified protein whose expression is upregulated in multiple cell lines and primary breast cancer tumors. Increased ERRF expression was significantly associated with ER or progesterone receptor (PR) positivity even in HER2-negative tumors. This higher ERRF expression correlated with better disease-free survival and overall survival. In two ER-positive breast cancer cell lines, knockdown of ERRF suppressed cell growth in vitro and tumorigenesis in xenograft models, suggesting that ERRF plays a role in estrogen-ER-mediated breast cancer cell growth and may thus be a potential therapeutic target.1) Su D, Fu X, Fan S, et al. Role of ERRF, a novel ER-related nuclear factor, in the growth control of ER-positive human breast cancer cells. Am. J. Pathol. 2012; 180:1189-201. |
| Immunogen | ERRF antibody was raised against a 15 amino acid peptide near the carboxy terminus of human ERRF . The immunogen is located within the last 50 amino acids of ERRF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ERRF Antibody can be used for detection of ERRF by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C1orf64 |
|---|---|
| Alternative Names | ERRF Antibody: ERRF Uncharacterized protein C1orf64 |
| Molecular Weight(MW) | Predicted: 19 kDa Observed: 15 kDa |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of ERRF in HeLa cells with ERRF antibody at 2.5 μg/ml.
Application
Immunofluorescence of ERRF in HeLa cells with ERRF antibody at 20 μg/ml.
Application
Western blot analysis of ERRF in (A) HeLa and (B) A-20 cell lysate with ERRF antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ERRF Antibody can be used for detection of ERRF by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ERRF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ERRF Antibody is supplied in PBS containing 0.02% sodium azide. |
