Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with ESPN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ESPN Antibody in 293 cell line lysates (35ug/lane)Application
Western Blot at 1:1000 dilution + SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ESPN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Espin, Autosomal recessive deafness type 36 protein, Ectoplasmic specialization protein, ESPN, DFNB36 |
| Product description | ESPN is a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells.1) Boulouiz,R., et.al., Am. J. Med. Genet.146A (23), 3086-3089 (2008) |
| Immunogen | This ESPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human ESPN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESPN |
|---|---|
| Alternative Names | Espin Autosomal recessive deafness type 36 protein Ectoplasmic specialization protein ESPN DFNB36 |
| Molecular Weight(MW) | 92 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with ESPN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ESPN Antibody in 293 cell line lysates (35ug/lane)
Application
Western Blot at 1:1000 dilution + SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
