Application
Immunohistochemistry staining of ESR1 in breast tissue using ESR1 monoclonal Antibody.| Product Name | ESR1 Antibody, Monoclonal |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ESR1, Era, ERalpha, Estrogen receptor, Estradiol receptor, ER-alpha, Estrogen receptor 1, NR3A1, ER, ESR, ESRA, Estrogen receptor alpha |
| Product description | Estrogen receptor alpha, a NR3 Steroid Receptor, activates transcription of a wide variety of genes in response to estrogen, particularly in reproductive tissues. Recent studies have shown that ER alpha also can function in a ligand-independent and DNA binding- independent manner, as well as through cell-surface signaling. ER alpha functions have also been demonstrated via the selective ER modulator (SERM) ligands and their various ER signaling pathways. ER alpha-knockout mice (alphaERKO) experience incomplete infertility and exhibit estrogen insensitivity in the reproductive tract, hypergonadotropic-hypogonadism, lack of pubertal mammary gland development, excess adipose tissue, testicular degeneration, and epididymal dysfunction. In the adult, estrogen is important in maintaining the constancy of bone mass through its effects on remodeling and bone turnover; inactivation of ER alpha is associated with low bone-mineral density and potentially could lead to osteoporosis. ER alpha exhibits complex patterns of alternative splicing and promoter usage, and at least 20 mRNA splice variants have been documented in normal and neoplastic tissues and cell lines. However, only two ER alpha protein isoforms have been documented: a common 66- kDa form and a truncated 46- kDa form that lacks the first 173 amino acids at the N terminus. |
| Immunogen | ESR1 monoclonal antibody was raised against Ni-NTA purified recombinant human ER-alpha (410-592aa) expressed in E. Coli strain BL21 (DE3) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| ESR1 antibody can be used in Western Blot, and immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ESR1 |
|---|---|
| Alternative Names | ESR1 Era ERalpha Estrogen receptor Estradiol receptor ER-alpha Estrogen receptor 1 NR3A1 ER ESR ESRA Estrogen receptor alpha |
Application
Immunohistochemistry staining of ESR1 in breast tissue using ESR1 monoclonal Antibody.| Application Notes | ESR1 antibody can be used in Western Blot, and immunohistochemistry starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store ESR1 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, pH 7.4, 0.03% sodium azide. |
