Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | ESR2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ESR2, Erb, ESRB, ESTRB, NR3A2, ER-BETA, ESR-BETA |
| Product description | ESR2 is a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.1) Brandenberger, A.W., et al., (1997) J. Clin. Endocrinol. Metab. 82 (10), 3509-3512. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ESR2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ESR2 antibody can be used for detection of ESR2 by ELISA at 1:312500. ESR2 antibody can be used for detection of ESR2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ESR2 |
|---|---|
| Alternative Names | ESR2 Erb ESRB ESTRB NR3A2 ER-BETA ESR-BETA |
| Molecular Weight(MW) | 48 kDa, 55 kDa, 55 kDa, 59 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ESR2 antibody can be used for detection of ESR2 by ELISA at 1:312500. ESR2 antibody can be used for detection of ESR2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ESR2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
