ERRs, which are coexpressed with ERs in prostatic cells, could regulate cell growth and modulate ER-mediated pathways via interference on ERalpha transcription in prostatic cells. Not only PNRC2 but also the corepressor TLE1 functioned as ERRgamma coactivator in a reporter gene analysis. Transcriptional activation by ERR3 can be ligand-independent.1) Hentschke, M., et al., (2003) Biochem. Biophys. Res. Commun. 312 (4), 975-982.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ESRRG.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
IHCWB
ESRRG antibody can be used for detection of ESRRG by ELISA at 1:62500. ESRRG antibody can be used for detection of ESRRG by western blot at 0.5&micr μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human Jurkat 0.5 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
ESRRG antibody can be used for detection of ESRRG by ELISA at 1:62500. ESRRG antibody can be used for detection of ESRRG by western blot at 0.5&micr μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ESRRG antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt